Biolegend, 117422, PE/Cyanine7 anti-mouse CD278 (ICOS) Antibody, 100μg

The 7E.17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand, B7h/B7RP-1 and B7-H2 is constitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse ICOS cDNA and ICOS hexahistidine fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF™ format is suggested for blocking studies.
Application References(PubMed link indicates BioLegend citation): Akbari O, et al. 2002. Nat. Med. 8:1024. Harada H, et al. 2003. J. Clin. Invest. 112:234. McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block) Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
Product Citations: Tian M, et al. 2021. Elife. 10:. PubMed
RRID: AB_2860636 (BioLegend Cat. No. 117421) AB_2860637 (BioLegend Cat. No. 117422)
Structure: CD28/CTLA-4, 47-57kD
Distribution: Activated T cells, subset of thymocytes
Function: Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor: B7h/B7RP-1/GL-50
Cell Type: T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544. 2. McAdam AJ, et al. 2000. J. Immunol. 165:5035. 3. Mak TW, et al. 2003. Nat. Immunol. 4:765
Gene ID: 54167
UniProt: View information about CD278 on UniProt.org
Clone: 7E.17G9
Regulatory Status: RUO
Other Names: Inducible COStimulatory molecule, H4
Isotype: Rat IgG2b, κ