Biolegend, 122303, APC anti-mouse DLK1 Antibody, 25μg

The Delta-Notch pathway controls developmental processes including cell fate determination, terminal differentiation, and proliferation. In mammals, four Notch receptors (NOTCH1–4) and five activating canonical ligands (JAGGED1, JAGGED2, DLL1, DLL3 and DLL4) have been described. Delta-like 1 homolog (DLK1) is a noncanonical Notch ligand, and acts as an inhibitor of Notch signaling. The inhibitory effect of DLK1 and DLK2 on Notch signaling may be mediated by competition with canonical ligands for the receptor binding site. Widely expressed during embryonic development, expression becomes restricted in the adult to neuroendocrine tissues like the pituitary gland, adrenal glands, pancreas, monoaminergic neurons in the central nervous system, testes, prostate, and ovaries.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: mDLK1-RBL1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
RRID: AB_3097344 (BioLegend Cat. No. 122303) AB_3097344 (BioLegend Cat. No. 122304)
Structure: Single-pass type I membrane protein
Distribution: Cytoplasm, plasma membrane
Function: Neuroendocrine differentiation, inhibits adipocyte differentiation
Interaction: Notch receptors
Ligand/Receptor: Notch receptors
Cell Type: Fibroblasts, Neurons
Biology Area: Neuroscience, Signal Transduction
Antigen References: Smas C.M, et al. 1993. Cell. 73:725-34. Laborda J, et al. 1993. J Biol Chem. 268:3817-20. Montalban-Loro R, et al. 2021. Proc Natl Acad Sci USA. 118 (11). Zhang L, et al. 2021. Stem Cells. 39:306-17. Ichinose M, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 320:G506-20.
Gene ID: 13386
UniProt: View information about DLK1 on UniProt.org
Clone: W21075D
Regulatory Status: RUO
Other Names: Pref, protein delta homolog 1
Isotype: Rat IgG2b, κ