Biolegend, 123415, PerCP/Cyanine5.5 anti-mouse CD21/CD35 (CR2/CR1) Antibody, 25μg

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD35/CFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Boackle S, et al. 2001 Immunity 15:775. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed Alexandre YO, et al. 2022. STAR Protoc. 3:101923. PubMed Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed Manzl C, et al. 2012. Cell Death Differ. 18:54. PubMed Na B, et al. 2016. PLoS One. 11: 0156429. PubMed Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Steinel N, et al. 2014. J Immunol. 193:2881. PubMed Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
RRID: AB_1595595 (BioLegend Cat. No. 123415) AB_1595490 (BioLegend Cat. No. 123416)
Structure: 145 kD/190 kD type I transmembrane glycoprotein
Distribution: B cells, follicular dendritic cells
Function: Signal transduction
Ligand/Receptor: Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type: B cells, Dendritic cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.
Gene ID: 1290212946
UniProt: View information about CD21/CD35 on UniProt.org
Clone: 7E9
Regulatory Status: RUO
Other Names: CR2/CR1
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.