Biolegend, 124625, Pacific Blue™ anti-mouse CD40 Antibody, 25μg

CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34 + hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD40 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 &124628) with a low endotoxin limit (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835. Bourgeois C, et al. 2002. Science 297:2060.
Product Citations: Solier S, et al. 2023. Int J Mol Sci. 24:. PubMed Sengupta A, et al. 2024. Vaccines (Basel). :12. PubMed Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed Zhao F et al. 2018. Immunity. 48(1):147-160 . PubMed Feng S, et al. 2021. Exp Ther Med. 21:20. PubMed
RRID: AB_2561475 (BioLegend Cat. No. 124625) AB_2561476 (BioLegend Cat. No. 124626)
Structure: 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
Distribution: Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.
Function: Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
Ligand/Receptor: CD154 on T cells
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.
Gene ID: 21939
UniProt: View information about CD40 on UniProt.org
Clone: 3/23
Regulatory Status: RUO
Other Names: Bp50, TNFRSF5
Isotype: Rat IgG2a, κ