Biolegend, 126701, Purified anti-mouse/human Galectin-3 (Mac-2) Antibody, 50μg

Galectin-3 (galactose-specific lectin 3) is a glycoprotein, also known as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and εBP. It is a member of animal β-galactoside-binding protein family that is expressed in cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3 contains carbohydrate recognition domains and binds to β-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Galectin-3 structurely possesses NWGR motif that is conserved in BH-1 domain of BCL-2 family and functions as an anti-apoptotic molecule.
50μg
Verified Reactivity: Mouse, Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Recombinant (partial), amino acids 151-251
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, a concentration of < 0.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Based on immunogen information, clone Gal397 may recognize African Green Baboon and Cynomolgus. It is recommended to test the reagent for optimal performance for other species. Due to in-house testing, the purified format is not recommended for flow cytometry and intracellular flow cytometry.
Product Citations: Bülck C, et al. 2023. Sci Adv. 9:eadf4055. PubMed Pang W, et al. 2023. iScience. 26:106579. PubMed Palau V, et al. 2021. Int J Mol Sci. 23:. PubMed Palau V, et al. 2021. Int J Mol Sci. 22:. PubMed Joeh E, et al. 2021. Curr Protoc. 1:e104. PubMed Feng T, et al. 2020. EMBO Rep. 21:e50219. PubMed Du H, et al. 2021. Life Sci Alliance. 4: . PubMed
RRID: AB_1134255 (BioLegend Cat. No. 126701) AB_1134256 (BioLegend Cat. No. 126702)
Structure: Human Galetcin-3 has 250 amino acids with a predicted molecular weight of 26 kD.Mouse Galetcin-3 has 264 amino acids with a predicted molecular weight of 27.5 kD.
Distribution: Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, dendritic cells
Function: Regulate cell proliferation and differentiation, adhesion, anti-apoptosis
Ligand/Receptor: Variety ligands, such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, etc
Cell Type: Dendritic cells, Epithelial cells, Macrophages, Monocytes
Biology Area: Cell Adhesion, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules
Antigen References: Kim HRC, et al. 1999. Cancer Res. 59:4148 Reljic R, et al. 2004. Immunol. Lett. 93:51. Takenaka Y et al. 2004. Mol. and Cell. Biol. 24:4395 Joo GH, et al. 2001. J. Leukoc. Biol. 69:555 Llinas L, et al. 2011. Immunol. Lett. 134:113-21 Cabezón R, et al. 2011. Immunol. Lett. 134:167-73
Gene ID: 168543958
UniProt: View information about Mac-2 on UniProt.org
Clone: Gal397
Regulatory Status: RUO
Other Names: Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Isotype: Mouse IgG1, κ