Biolegend, 127022, PerCP/Cyanine5.5 anti-mouse CD317 (BST2, PDCA-1) Antibody, 100μg

CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified, IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse plasmacytoid dendritic cells (DCs)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.
Application References(PubMed link indicates BioLegend citation): Blasius AL, et al. 2006. J. Immunol. 177:3260. Schliemann C, et al. 2010. Blood 115:736. (FA, IF) Rajagopal D, et al. 2010. Blood 115:1949. (Depletion) Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion) Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed
Product Citations: Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
RRID: AB_2566646 (BioLegend Cat. No. 127021) AB_2566647 (BioLegend Cat. No. 127022)
Structure: Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
Distribution: Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.
Function: Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
Cell Type: Dendritic cells
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Douglas JL. et al. 2009. J Virol. 83(16):7931 2. Cao W et al. 2009. J. Exp. Med. 206(7):1603 3. Neil SJ. et al. 2008. Nature 451:425
Gene ID: 69550
UniProt: View information about CD317 on UniProt.org
Clone: 927
Regulatory Status: RUO
Other Names: BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Isotype: Rat IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.