Biolegend, 127102, Purified anti-mouse CD317 (BST2, PDCA-1) Antibody, 100μg

CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified as IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse plasmacytoid dendritic cells (DCs)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Blasius AL, et al. 2006. J. Immunol. 177:3260Cocka LJ, et al. 2012. PLoS Pathog. 8:e1002931. PubMed.
Product Citations: Cocka L, Bates P 2012. PLoS One. 8:e1002931. PubMed Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed Olety B, et al. 2021. Sci Adv. 7:eabj7398. PubMed
RRID: AB_1953279 (BioLegend Cat. No. 127102)
Structure: Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
Distribution: Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.
Function: Recently identified as antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
Cell Type: Dendritic cells, B cells
Biology Area: Immunology, Innate Immunity, Costimulatory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Douglas JL. et al. 2009. J Virol. 83(16):79312. Cao W et al. 2009. J. Exp. Med. 206(7):16033. Neil SJ. et al. 2008. Nature 451:425
Gene ID: 69550
UniProt: View information about CD317 on UniProt.org
Clone: 129C1
Regulatory Status: RUO
Other Names: BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Isotype: Rat IgG2b, κ