Biolegend, 127404, Biotin anti-mouse Podoplanin Antibody, 500μg

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.
Application References(PubMed link indicates BioLegend citation): Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. Farr AG, et al. 1992. J. Exp. Med. 176:1477. Bekiaris V, et al. 2008. J. Immunol. 180:6768. Algars A, et al. 2011. Blood 117:4387. PubMed Reis VO, et al. 2012. Immunobiology. 217:831. PubMed Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC) Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Auciello FR, et al. 2019. Cancer Discov. 9:617. PubMed Helms EJ, et al. 2021. Cancer Discov. Online ahead of print. PubMed Saunders A, et al. 2014. J Immunol. 193:2831. PubMed Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed Rodda L, et al. 2015. J Immunol. 195: 4781 - 4791. PubMed Progatzky F, et al. 2021. Nature. 599:125. PubMed Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed Ruggeri JM, et al. 2020. Am J Pathol. 190:1735. PubMed Rodriguez AB, et al. 2022. Front Immunol. 13:1009306. PubMed Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed Abe J, et al. 2014. J Immunol. 193:1363. PubMed
RRID: AB_1134221 (BioLegend Cat. No. 127403) AB_1134222 (BioLegend Cat. No. 127404)
Structure: 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution: Expressed on epithelial and mesothelial cells.
Cell Type: Epithelial cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References: 1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. 2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.
Gene ID: 14726
UniProt: View information about Podoplanin on UniProt.org
Clone: 8.1.1
Regulatory Status: RUO
Other Names: T1a, gp38, stromal cell marker
Isotype: Syrian Hamster IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.