Biolegend, 127406, Alexa Fluor® 488 anti-mouse Podoplanin Antibody, 100μg

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.
Application References(PubMed link indicates BioLegend citation): Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. Farr AG, et al. 1992. J. Exp. Med. 176:1477. Bekiaris V, et al. 2008. J. Immunol. 180:6768. Algars A, et al. 2011. Blood 117:4387. PubMed Reis VO, et al. 2012. Immunobiology. 217:831. PubMed Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC) Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed Du Y, et al. 2022. FASEB J. 36:e22248. PubMed Hoorweg K, et al. 2015. J Immunol. 195: 4257 - 4263. PubMed Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed Fujikura D, et al. 2013. PLoS One. 8:e55321. PubMed Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed Nadafi R, et al. 2020. Cell Reports. 30(12):4110-4123. PubMed Jung M, et al. 2016. Sci Signal. 9: ra64. PubMed Durré T, et al. 2018. Nat Commun. 9:5178. PubMed
RRID: AB_1133992 (BioLegend Cat. No. 127405) AB_2161930 (BioLegend Cat. No. 127406)
Structure: 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution: Expressed on epithelial and mesothelial cells.
Cell Type: Epithelial cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References: 1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. 2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.
Gene ID: 14726
UniProt: View information about Podoplanin on UniProt.org
Clone: 8.1.1
Regulatory Status: RUO
Other Names: T1a, gp38, stromal cell marker
Isotype: Syrian Hamster IgG