Biolegend, 127707, PE anti-mouse TCR Vα8.3 Antibody, 25μg

The B21.14 antibody recognizes an epitope of the CDR1 on the Va 8.3 subfamily of TCR having the a,b,c and d haplotypes of Tcra gene complex.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Soluble TCR from mouse CTL clone CW3/1.1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Necker A, et al. 1991. Eur J. Immunol. 21:3035 Brodnicki TC, et al. 1996. Mol Immunol 33:253 Tomonari K, et al. 1992. Immunogenetics 35:291
Product Citations: Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
RRID: AB_1186072 (BioLegend Cat. No. 127708)
Structure: TCR has a two chain structure, consisting of alpha and beta chain. It belongs to Ig superfamily.
Distribution: On a subset of T cells
Function: Recognize MHC/peptide complex on the antigen-presenting cells.
Interaction: Associate with CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor: TCR recognizes MHC/peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Kubo RT, et al. 1989. J. Immunol. 142:2736 2. Brodnicki TC, et al. 1996. Mol Immunol 33:253
Gene ID: 110469
UniProt: View information about TCR Valpha8.3 on UniProt.org
Clone: B21.14
Regulatory Status: RUO
Other Names: T cell receptor Vα 8.3
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.