Biolegend, 127807, PE anti-mouse TCR Vα2 Antibody, 25μg

The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Soluble TCR from mouse CTL clone KB5-C20
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.
Application References(PubMed link indicates BioLegend citation): Pircher H, et al. 1992. Eur. J. Immunol.. 22:399. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed Rao RR, et al. 2012. Immunity. 36:374. PubMed.
Product Citations: Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Gargaro M, et al. 2022. Immunity. 55:1032. PubMed Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed Sartorius R, et al. 2015. EMBO Mol Med. 7: 973 - 988. PubMed Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed Huang J, et al. 2014. J Immunol. 192:1972. PubMed Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed Bahmani B, et al. 2018. J Clin Invest. 128:4770. PubMed Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
RRID: AB_1134184 (BioLegend Cat. No. 127807) AB_1134183 (BioLegend Cat. No. 127808)
Distribution: On a subset of T cells
Function: In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
Interaction: Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor: TCR recognizes MHC/peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Kubo RT, et al. 1989. J. Immunol.. 142:2736. 2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.
Gene ID: 21473
UniProt: View information about TCR Valpha2 on UniProt.org
Clone: B20.1
Regulatory Status: RUO
Other Names: T cell receptor Vα 2
Isotype: Rat IgG2a, λ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.