Biolegend, 128904, Alexa Fluor® 647 anti-mouse TCR Vβ10 (a haplotype) Antibody, 100μg

This antibody reacts with Vb10 TCR of mice having the a haplotype (SJL, C57BR, SWR, C57L), but not with TCRb (e.g., AKR, BALB/c C3H, C57BL, DBA/1, DBA/2) or TCRc haplotypes (e.g., RIII).
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: 1B6 Rag2 knockout transgenic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
RRID: AB_1236445 (BioLegend Cat. No. 128903)
Structure: TCR consists of alpha and beta chain. This antibody recognizes Vb 10 of TCR a haplotype (e.g. SJL, C57L, SWR and C57BR).
Distribution: Positive on a subset of alpha/beta T cells from mice of TCR a haplotype.
Ligand/Receptor: MHC/peptide
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Necker A, et al. 1991. Eur. J. Immunol. 21:3035
Gene ID: 100124660
UniProt: View information about TCR Vbeta10 on UniProt.org
Clone: 8.48
Regulatory Status: RUO
Other Names: T cell receptor Vb10
Isotype: Mouse IgG1, κ