Biolegend, 129610, Pacific Blue™ anti-mouse Siglec H Antibody, 100μg

The Siglec-H is a member of CD33-related Siglec family which mostly expressed in the innate immune system. Siglec-H is reported to be specifically expressed on plasmacytoid dendritic cells. Siglec-H depends on adaptor protein DAP 12 (DNAX-activating protein of 12 kD) for surface expression and signal transduction. Cross-linking with this antibody can selectively inhibit interferon-alpha production by IPCs following TLR9 ligation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Siglec-H-Fc recombinant fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Blasius AL, et al. 2006. J. Immunol. 177:3260.
Product Citations: Forbester JL, et al. 2020. J Virol. 94:. PubMed Bi E, et al. 2018. J Clin Invest. 128:4821. PubMed
RRID: AB_10643868 (BioLegend Cat. No. 129609) AB_10643994 (BioLegend Cat. No. 129610)
Structure: Sialic acid-binding immunoglobulin-like lectins
Distribution: Siglec-H is expressed exclusively on natural interferon-producing cells (IPCs), also called plasmacytoid dendritic cells in spleen, blood and lymph nodes. The IPCs are characterized by a complex phenotype (CD11c+, Ly-6C+, B220+, CD11b-, CD8+/-).
Function: Siglec-H associates with adaptor protein DAP12 for cell signaling, which reduces type 1 IFN response of IPC to CpG.
Ligand/Receptor: Sialic acid
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: 1. Blasius AL, et al. 2006. Blood 107:2474. 2. Zhang J, et al. 2006. Blood 107:3600. 3. Blasius AL, et al. 2007. J. Exp. Med. 204:2561.
Gene ID: 233274
UniProt: View information about Siglec H on UniProt.org
Clone: 551
Regulatory Status: RUO
Other Names: Sialic acid-binding immunoglobulin-like lectin, SiglecH
Isotype: Rat IgG1, κ