Biolegend, 131218, Brilliant Violet 421™ anti-mouse CD194 (CCR4) Antibody, 50μg

Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85% identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naïve T cells, bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes and express cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity to migrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play an important role in the ligand-mediated recruitment of T cells into the skin in mice and humans, specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site of injury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucial for the induction and maintenance of self-tolerance and are present in peripheral tissues such as skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor and its ligand CCL22 (5).
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Saito K, et al. 2008. J. Immunol. 181:6889. PubMed Ueha S, et al. 2007. J. Leukoc. Biol. 82:1230. PubMed 3. Sharma R, et al. 2009 J. Immunol. 183:1065 (FC) PubMed Dogan R, et al. 2011. J. Leukoc. Biol. 89:93. PubMed Liu H, et al. 2011. Sci Transl Med. 3:82. PubMed. Shankar SP, et al. 2012. J Immunol. 188:6347. PubMed.
Product Citations: Xiong Y, et al. 2016. J Immunol. 196: 2526 - 2540. PubMed Stathopoulou C, et al. 2020. Immunity. 49(2):247-263.e7.. PubMed Herrero–Cervera A, et al. 2019. Diabetologia. 62:2143. PubMed Qadir AS, et al. 2021. iScience. 24:103348. PubMed Jeljeli M, et al. 2021. Front Immunol. 12:670776. PubMed
RRID: AB_10933253 (BioLegend Cat. No. 131217) AB_2650890 (BioLegend Cat. No. 131218)
Structure: GPCR, chemokine receptor, seven transmembrane receptor
Distribution: Memory T cells, macrophages, platelets, basophils, Th2 cells, and Tregs
Function: CCR4 and its ligands (CCL17 and CCL22) are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
Interaction: Interaction between CCR4 and its ligand TARC/CCL17 on activated endothelial cells mediates T cell extravasation by stimulating integrin-dependent adhesion
Ligand/Receptor: CCl17 and CCL22
Cell Type: Basophils, Embryonic Stem Cells, Macrophages, Platelets, T cells, Th2, Tregs
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Biederman T, et al. 2002. Eur. J. Immun. 32:3171. 2. Mirshahpanah P, et al. 2008. Exp. Dermatol. 17:30. 3. Kusumoto M, et al. 2007. J. Interferon Cytokine Res 27:901. 4. Clark RA and Kupper TS. 2006. Blood 109:194. 5. Lee I, et al. 2005. J. Exp. Med. 201:1037.
Gene ID: 12773
UniProt: View information about CD194 on UniProt.org
Clone: 2G12
Regulatory Status: RUO
Other Names: CC CKR-4, CHEMR1, Cmkbr4, LESTR, MGC151418, Sdf1r
Isotype: Armenian Hamster IgG
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.