Biolegend, 131902, Purified anti-mouse IL-21R Antibody, 500μg

The IL-21R is a member of the cytokine receptor family that closely resembles IL-2R beta and IL-4R alpha. IL-21R is expressed by all mature lymphocytes and up-regulated after B and/or T cell activation, with the highest expression by activated B cells. A functional IL-21R consists of the IL-21R subunit and the commone gamma-chaiin. Upon binding to IL-21, it activates Jak-1, Jak-3, STAT1, STAT3 and STAT 5 transduction pathway.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Rat YB2/0 hybridoma transfectant cell line expressing truncated IL-21R
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Jin H, et al. 2004. J. Immunol. 173:657. (FC) Jin H and Malek T. 2006. J. Leukocyte Biol. 80:1416. (FC IP)
Product Citations: Park JY, et al. 2022. Cell Rep. 38:110219. PubMed
RRID: AB_1279435 (BioLegend Cat. No. 131902)
Structure: A member of the class 1 cytokine receptor family. Show significant sequence and structural homology with the IL-4R alpha chain.
Distribution: Express on all mature lymphocytes including B cells, T cells, and NK cells. Reported to be up-regulated upon activation and highest expression on follicular B cells.
Ligand/Receptor: IL-21
Cell Type: B cells, Lymphocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1.Mehta DS, et al. 2004. Immu Reviews 202:84
Gene ID: 60504
UniProt: View information about IL-21R on UniProt.org
Clone: 4A9
Regulatory Status: RUO
Other Names: IL-21 Receptor
Isotype: Rat IgG2a, κ