Biolegend, 133307, Biotin anti-mouse Lineage Panel, 500tests

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. This product includes individual vials of the following biotin antibodies: anti-mouse CD3ε, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid Cells, clone TER-119. Materials provided: 1. 500 tests (1 mL) each of: Biotin anti-mouse CD3ε, Biotin anti-mouse Ly-6G/Ly-6C (Gr-1), Biotin anti-mouse CD11b, Biotin anti-mouse CD45R/B220, Biotin anti-mouse Ter-119/Erythroid Cells Materials not provided:
500tests
Verified Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solutions should be stored undiluted at 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested Magnetic particle separation - Verified
Recommended Usage: Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 2 µl of each antibody per million cells or 2 µl of each antibody per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Morris SJ, et al. 1997. Development. 124(10):1929-1939 Okada S, et al. 1992. Blood180 (12):3044-3050 Spangrude GJ, et al. 1988. Science 241 (4861):58-62 Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926 Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed Ito R, et al. 2012. Exp Hematol. 40:953. PubMed.
Product Citations: Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed Chudakov DB, et al. 2022. Vaccines (Basel). 10: . PubMed Li X, et al. 2022. Cell. 185:1709. PubMed Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed Bonnet C, et al. 2021. iScience. 24:103399. PubMed Grizotte–Lake M, et al. 2018. Immunity. 49:1103. PubMed LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed de Almeida MJ et al. 2017. Cell stem cell. 21(6):725-729 . PubMed Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed Morimoto Y, et al. 2018. Immunity. 49:134. PubMed Freter R, et al. 2021. Nat Commun. 12:3318. PubMed Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed Chen C, et al. 2020. Cell Rep. 2136:30. PubMed Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed Guo L, et al. 2020. Nat Commun. 0.972222222. PubMed Li X, et al. 2019. Stem Cells. 37:937. PubMed Lion M, et al. 2020. Proc Natl Acad Sci U S A. 117:5453. PubMed
RRID: AB_11124348 (BioLegend Cat. No. 133307)
Distribution: T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes
Biology Area: Immunology
Antigen References: 1. Morrison SJ, et al. 1997. Development 124:1929. 2. Okada S, et al. 1992. Blood 80:3044. 3. Spangrude GJ, et al. 1988. Science 241:58. 4. Spangrude GJ, et al. 1990. Exp Hematol. 18:920. 5. Tan SL, et al. 2006. J. Immunol. 176:2872.
Gene ID: 104231
UniProt: View information about Lineage on UniProt.org
Clone: 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status: RUO
Other Names: Lin-1
Isotype: Armenian Hamster IgG/Rat IgG2a/Rat IgG2b
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.