Biolegend, 134103, PE anti-mouse CD268 (BAFF-R) Antibody, 25μg

BAFF (B cell-activating factor belonging to the TNF family) receptor (BAFF-R) is one of the three receptors that bind to BAFF. It is a type III transmembrane TNF receptor family member. BAFF-R is expressed at high levels in resting B cells and at lower levels in activated B cells, a small subset of activated/memory CD4 + T cells. It is a major mediator of BAFF dependent co-stimulatory response in peripheral B and T cells. It was reported that the mice expressing defective BAFF-R have disrupted B cell maturation. BAFF receptor appears to be particularly important for the regulation of normal B cell survival and maturation, as well as in the pathophysiology of aggressive B-cell malignancies and autoimmune diseases.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse BAFF receptor transfected rat RBL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Ng LG, et al. 2006. Eur. J. Immunol. 36:1837. Ng LG, et al. 2004. J Immunol. 173:807. Batten M, et al. 2000. J. Exp. Med. 192:1453. Fu L, et al. 2009. Blood 113:4627.
Product Citations: Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed Silva N, et al. 2016. J Immunol. 196: 2591 - 2601. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID: AB_1626160 (BioLegend Cat. No. 134103) AB_2203552 (BioLegend Cat. No. 134104)
Structure: A type III transmembrane TNF receptor family member
Distribution: Expressed on most B cells, a small subset of CD4+ T cells.
Function: Play an importance role in B cell development, maturation, and survival.
Ligand/Receptor: BAFF (BLyS)
Cell Type: B cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Thompson JS, et al. 2001. Science 293:2108. 2. Schiemann B, et al. 2001. Science 293:2111. 3. Batten M, et al. 2000. J. Exp. Med. 192:1453. 4. Fu L, et al. 2009. Blood 113:4627.
Gene ID: 72049
UniProt: View information about CD268 on UniProt.org
Clone: 7H22-E16
Regulatory Status: RUO
Other Names: BR3, BAFFR, BAFF receptor, TNFRSF13C, B cell-activating factor receptor, BLys receptor 3
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.