Biolegend, 135207, PerCP/Cyanine5.5 anti-mouse CD279 (PD-1) Antibody, 25μg

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3, in vivo blocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.
Application References(PubMed link indicates BioLegend citation): Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block) Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block) Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed Otano I, et al. 2021. Nat Commun. 12:7296. PubMed Hansel C, et al. 2019. Front Pharmacol. 10:244. PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed Utz B, et al. 2020. Sci Rep. 10:18733. PubMed Gozgit JM, et al. 2021. Cancer Cell. :. PubMed Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed Wu L, et al. 2020. Cell Rep. 33:108327. PubMed Hekim C, et al. 2017. Cancer Immunol Res. 5:157. PubMed Barbet G, et al. 2018. Immunity. 48:584. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed Truckenbrod EN, et al. 2021. Elife. 10:. PubMed Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed Guiteras J, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID: AB_10550092 (BioLegend Cat. No. 135207) AB_2159184 (BioLegend Cat. No. 135208)
Structure: A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
Distribution: Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.
Function: Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
Ligand/Receptor: B7-H1 (PD-L1) and B7-DC (PD-L2)
Cell Type: B cells, T cells
Biology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Nishimura H, et al. 2001. Science 291:319 2. Agata Y, et al. 1996. Int. Immunol. 8:765 3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706 4. Barber DL, et al. 2006. Nature 439:682 5. Keir ME, et al. 2005. J. Immunol. 175:7372 6. Koehn BH. et al. 2008. J Immunol. 181:5313
Gene ID: 18566
UniProt: View information about CD279 on UniProt.org
Clone: 29F.1A12
Regulatory Status: RUO
Other Names: PD-1, Programmed Death-1, PDCD1
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.