Biolegend, 135311, PE/Cyanine5 anti-mouse CD135 Antibody, 25μg

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Flt3 transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Sergejeva S, et al. 2004. Blood 103:1270. Auffray C, et al. 2009. J. Exp. Med. 206:595. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
Product Citations: Deák P, et al. 2022. Cell Rep. 41:111563. PubMed Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
RRID: AB_2107049 (BioLegend Cat. No. 135311) AB_2263031 (BioLegend Cat. No. 135312)
Structure: A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution: Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.
Function: Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor: Flk-2/FLT3 ligand
Cell Type: B cells, Hematopoietic stem and progenitors
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Waskow C, et al.Nat. Immunol. 9:676 2. Veiby OP, et al. 1996. Blood 88(4):1256 3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953 4. Mattews W, et al. 1991. Cell. 65(7):1143 5. Hannum C, et al. 1994. Nature 368(2):643 6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478
Gene ID: 14255
UniProt: View information about CD135 on UniProt.org
Clone: A2F10
Regulatory Status: RUO
Other Names: Flk-2, Flt3, Ly-72
Isotype: Rat IgG2a, κ