Biolegend, 135901, Purified anti-mouse CD140a Antibody, 25μg

Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse PDGFR-α-hIgG1 recombinant fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested WB, IHC-P, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.
Application References(PubMed link indicates BioLegend citation): Takakura N, et al. 1996. J. Invest. Dermatol. 107:770. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block) Chen H, et al. 2015. ASN Neuro 8:7. PubMed. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)
Product Citations: Kim J, et al. 2016. PLoS One. 11: 0158067. PubMed Dittmer M, et al. 2018. Mol Brain. 11:25. PubMed Engelbrecht E, et al. 2022. Microcirculation. 29:e12752. PubMed Weng L, et al. 2016. Sci Rep. 6:38632. PubMed Albrecht S, et al. 2016. PLoS One. 11:e0163841. PubMed Paulo E, et al. 2018. Sci Rep. 8:11001. PubMed Jarjour AA, et al. 2020. Glia. 68(9):1840-1858. PubMed Gibson EM, et al. 2019. Cell. 176:43:00. PubMed Ghelman J, et al. 2021. Glia. 69(11):2699-2716. PubMed Chen H, et al. 2015. ASN Neuro. 8:7. PubMed Liu M, et al. 2018. Diabetologia. 61:2333. PubMed Shin J, et al. 2018. Diabetes. 67:1068. PubMed Yoshioka K, et al. 2020. Front Cell Dev Biol. 0.884027778. PubMed Yin W, et al. 2022. Development. 149:. PubMed Iida Y, et al. 2020. J Immunother Cancer. 8:. PubMed Maller O, et al. 2020. Nat Mater. 20:548. PubMed
RRID: AB_1953327 (BioLegend Cat. No. 135901) AB_1953328 (BioLegend Cat. No. 135902)
Structure: Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution: Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
Function: Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand/Receptor: PDGFs
Cell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551 2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124 3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883
Gene ID: 18595
UniProt: View information about CD140a on UniProt.org
Clone: APA5
Regulatory Status: RUO
Other Names: PDGF receptor-α, PDGFR-α
Isotype: Rat IgG2a, κ