Biolegend, 136517, Brilliant Violet 650™ anti-mouse CD93 (AA4.1, early B lineage) Antibody, 50μg

CD93 is a 130-140kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen. It is a receptor expressed on immature B lymphocytes, hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, embryonic yolk sac. CD93 expression level on splenic immature/transitional B cells is much lower than in bone marrow. It is reinduced during plasma cell differentiation and plays an important role in maintaining plasma cells in bone marrow niches. Immature dendritic cells express CD93 and down-regulate this molecule upon maturation, suggesting a role in uptake of particles by DC. It is also expressed on monocytes, macrophages, and endothelial cells. Macrophages from CD93 (-/-) mice had a significant phagocytic defect in the clearance of apoptotic cells in vivo , indicating CD93 may contribute to the in vivo clearance of dying cells. Binding of CD93 to C1q remains controversial.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Pre-B lymphoma 70Z/3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): McKearn JP, et al. 1984. J. Immunol. 132:332 Hanihara-Tatsuzaawa F, et al. 2014. J Biol Chem. 289:30925. PubMed
RRID: AB_3698911 (BioLegend Cat. No. 136517)
Structure: A 130-140 kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen.
Distribution: Expressed during immature B lymphocytes, hematopoietic progenitors and stem cells in bone marrow, immature dendritic cells, monocytes, macrophages, and endothelial cells.
Function: CD93 plays an important role in maintaining of plasma cells in bone marrow niches. It may play a role in uptake of particles by DC and the in vivo clearance of dying cells by macrophages.
Cell Type: B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Steinberger P, et al. 2002. J. Leukoc. Biol. 71:133 2. Chevrier S, et al. 2009. Proc. Nat. Acad. Sci. U. S. A. 106:3895 3. Norsworthy PJ, et al. 2004. J. Immunol. 172:3406 4. Li YS, et al. 1996. Immunity 5:527 5. Szilvassy SJ, et al. 1993. Blood 81:2310
Gene ID: 17064
UniProt: View information about CD93 on UniProt.org
Clone: AA4.1
Regulatory Status: RUO
Other Names: AA4.1, C1qRp, Early B lineage antigen
Isotype: Rat IgG2b, κ