Biolegend, 136703, PE anti-mouse CD256 (APRIL) Antibody, 50μg

CD256 (APRIL, TNFSF13, TALL2, TRDL1) a transmembranal protein of 26 kD, forms a homotrimeric molecule that is cleaved from the cell surface resulting in the soluble form of APRIL; it also forms heterotrimers with BLyS. Its receptor are TNFRSF13b/TACI and TNFRSF17/BCMA. Expressed by T cells, B cells, dendritic cells, monocytes, and macrophages, APRIL induces proliferation of T and B cells, promotes IgA class switching, downregulates T-cell dependent antibody responses, and regulates the growth of a variety of tumor cells.
50μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Fusion protein of murine Maltose binding protein (MBP) and soluble April
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Moreau J, et al. 2015. Blood. 126: 1184-1192. PubMed Wang W, et al. 2014. J Immunol. 192:3011. PubMed
RRID: AB_2206542 (BioLegend Cat. No. 136703) AB_2206541 (BioLegend Cat. No. 136704)
Structure: Transmembranal protein of 26 kD, forms a homotrimer that is cleaved from the cell surface resulting in the soluble form. forms heterotrimers with BLyS
Distribution: T cells, B cells, dendritic cells, monocytes, and macrophages
Function: Induces proliferation of T and B cells, promotes IgA class switching and downregulates T-cell dependent antibody responses, regulates the growth of a variety of tumor cells.
Ligand/Receptor: TNFRSF13b/TACI, TNFRSF17/BCMA
Bioactivity: Proliferation, apoptosis inhibition, IgA class switch.
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Belnoue E, et al. 2008 Blood 111:2755. 2. Xie P, et al. 2007. Immunity 27:253. 3. Mohr E, et al. 2009. J. Immunol. 182:2113. 4. Tezuka H, et al. 2007. Nature 448:92. 5. Benson MJ, et al. 2008. J. Immunol. 180:3655.
Gene ID: 69583
UniProt: View information about CD256 on UniProt.org
Clone: A3D8
Regulatory Status: RUO
Other Names: TNFSF13, TALL2, TRDL1
Isotype: Armenian Hamster IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.