Biolegend, 137012, Alexa Fluor® 488 anti-mouse CD68 Antibody, 100μg

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Purified Con A receptor glycoproteins from the P815 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested FC, 3D IHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.
Application References(PubMed link indicates BioLegend citation): Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB) Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC) Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC) Kayama H, et al. 2012. PNAS. 109:5010. PubMed Park S, et al. 2013. Biomaterials. 34:598. PubMed Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Barbour T, et al. 2016. Kidney Int. 89: 823-832. PubMed Mizutani T, et al. 2023. iScience. 26:106081. PubMed Fukuzaki Y, et al. 2023. iScience. 26:106340. PubMed Basta MD, et al. 2023. iScience. 26:106570. PubMed Zhou Y, et al. 2021. FASEB J. 35:e21288. PubMed Sun Z, et al. 2022. Br J Pharmacol. 179:1237. PubMed Chen O, et al. 2023. J Clin Invest. 133: . PubMed Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed Zhou Y, et al. 2019. Differentiation. 109:16:00. PubMed Wang LH, et al. 2021. Sci Adv. 7: . PubMed García L, et al. 2015. Exp Biol Med. 240: 904 - 916. PubMed DeDreu J, et al. 2020. FASEB J. 34:9316. PubMed Paterson N, et al. 2022. Elife. 11:. PubMed Lv F, et al. 2022. iScience. 25:103639. PubMed Vegas A, et al. 2016. Nat Biotechnol. 10.1038/nbt.3462. PubMed Kawasaki N, et al. 2013. Proc Natl Acad Sci U S A. 110:7826. PubMed Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed DeDreu J, et al. 2022. FASEB J. 36:e21995. PubMed Li C, et al. 2020. Cancer Immunol Res. 1.030555556. PubMed
RRID: AB_2074847 (BioLegend Cat. No. 137011) AB_2074846 (BioLegend Cat. No. 137012)
Structure: A member of the lysosomal-associated membrane protein (lamp) family.
Distribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
Function: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type: Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References: 1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833. 2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.
Gene ID: 12514
UniProt: View information about CD68 on UniProt.org
Clone: FA-11
Regulatory Status: RUO
Other Names: Macrosialin
Isotype: Rat IgG2a