Biolegend, 137206, PE anti-mouse/human Helios Antibody, 25tests

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is also involved in tumor immunity.
25tests
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Helios peptide (aa 51-107)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. It is recommended that the reagent be titrated for optimal performance for each application.For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear™ 10X Perm Buffer (Cat. No. 424403). View more applications data for a different format of this clone in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed Verhagen J and Wraith D. 2010. J. Immunol. 185:7129. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed Vaeth M, et al. 2012. PNAS. 109:16258. PubMed Angin M, et al. 2014. PLoS One. 9:86920. PubMed Bedke T, et al. 2014. Immunol Cell Biol. PubMed Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Product Citations: Hatam L, et al. 2012. Clin Cancer Res. 2.086805556. PubMed Takimoto Y, et al. 2023. iScience. 26:106220. PubMed Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed McClymont S, et al. 2011. J Immunol. 186:3918. PubMed Zeng Z, et al. 2016. J Exp Med. 213: 1079 - 1093. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Spallanzani R, et al. 2015. J Immunol. 195: 2141-2148. PubMed MaruYama T 2015. Biochem Biophys Res Commun. 464: 586-589. PubMed Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed Baine I, et al. 2013. J Immunol. 190:1008. PubMed Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed Trotta E, et al. 2018. Nat Med. 24:1005. PubMed Sawanobori Y, et al. 2021. Histochem Cell Biol. 156:133. PubMed Zeng Z, et al. 2013. J Immunol. 191:4184. PubMed Goodwin M, et al. 2020. Sci Adv. 6:eaaz0571. PubMed Dehzad N, et al. 2011. J Immunol. 186:2238. PubMed Wraith J 2010. J Immunol. 185:7129. PubMed Alhosaini K, et al. 2021. Brain Sci. :11. PubMed Van Gool F, et al. 2019. Immunity. 50:362. PubMed Paiva R, et al. 2013. Proc Natl Acad Sci U S A. 110:6494. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Yuan X, et al. 2017. Elife. 6:e29540. PubMed Ostadkarampour M, et al. 2016. PLoS One. 11:e0164751. PubMed Santoni de Sio FR, et al. 2018. J Allergy Clin Immunol. 142:1909. PubMed Redpath S, et al. 2015. Infect Immun . 83: 3881 - 3889. PubMed MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed Kim YC, et al. 2018. J Immunol. 201:1671. PubMed Baron CS, et al. 2018. Nat Commun. 9:2517. PubMed Adel–Patient K, et al. 2018. Clin Transl Allergy. 8:38. PubMed Zhao J, et al. 2011. J Exp Med. 208:1571. PubMed Grinberg–Bleyer Y, et al. 2017. Cell. 170:1096. PubMed
RRID: AB_10552903 (BioLegend Cat. No. 137206) AB_10660749 (BioLegend Cat. No. 137216)
Structure: A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution: Regulatory T cells (Tregs), conventional T cells, thymocytes.
Function: Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Kelly CM, et al. 1998. Curr. Biol. 8:508. 2. Dovat S, et al. 2005. J. Immunol. 175:3508. 3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167. 4. Cai Q, et al. 2009. J. Immunol. 183:2303. 5. Zhang Z, et al. 2007. Blood 109:2190. 6. Hahm K, et al. 1998. Genes Dev. 12:782.
Gene ID: 2277922807
UniProt: View information about Helios on UniProt.org
Clone: 22F6
Regulatory Status: RUO
Other Names: IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Isotype: Armenian Hamster IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.