Biolegend, 137220, Pacific Blue™ anti-mouse/human Helios Antibody, 100tests

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is also involved in tumor immunity.
100tests
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Helios peptide (aa 51-107)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear™ 10X Perm Buffer (Cat. No. 424403). View more applications data for a different format of this clone in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed Verhagen J and Wraith D. 2010. J. Immunol. 185:7129. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed Vaeth M, et al. 2012. PNAS. 109:16258. PubMed Angin M, et al. 2014. PLoS One. 9:86920. PubMed Bedke T, et al. 2014. Immunol Cell Biol. PubMed Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Product Citations: Danileviciute E, et al. 2022. Nat Metab. 4:589. PubMed Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed Schmitt E, et al. 2013. J Immunol. 191:5640. PubMed Wang L, et al. 2019. Cell Rep. 29:1848. PubMed Schoenbrunn A, et al. 2012. J Immunol. 189:5985. PubMed Hope C, et al. 2014. Kidney Int. 86:175. PubMed Merwe M, et al. 2013. J Immunol. 191:5746. PubMed Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed Kreiser S, et al. 2014. Immunobiology. 220:270. PubMed Singh K, et al. 2015. Sci Rep. 14:7767. PubMed Bouvy A, et al. 2014. Nephrol Dial Transplant. 29:1587. PubMed Yero A, et al. 2019. J Virol. 93:e00371-19. PubMed Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed Baptista A, et al. 2014. Elife. 3:4433. PubMed Esfahani K, et al. 2019. Nat Commun. 3.688888889. PubMed Watson MJ, et al. 2021. Nature. 591:645. PubMed Dhuban K, et al. 2015. J Immunol. 194:3687. PubMed Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed Hu M, et al. 2019. Nat Commun. 10:3031. PubMed Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed Serre K, et al. 2011. PLoS One. 6:e20731. PubMed Yang YH, et al. 2020. Cell Reports. 30(5):1515-1529.e4. PubMed Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed Chopp LB, et al. 2020. Immunity. 53(6):1182-1201.e8. PubMed Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
RRID: AB_10575625 (BioLegend Cat. No. 137210) AB_10690535 (BioLegend Cat. No. 137220)
Structure: A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution: Regulatory T cells (Tregs), conventional T cells, thymocytes.
Function: Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Kelly CM, et al. 1998. Curr. Biol. 8:508. 2. Dovat S, et al. 2005. J. Immunol. 175:3508. 3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167. 4. Cai Q, et al. 2009. J. Immunol. 183:2303. 5. Zhang Z, et al. 2007. Blood 109:2190. 6. Hahm K, et al. 1998. Genes Dev. 12:782.
Gene ID: 2277922807
UniProt: View information about Helios on UniProt.org
Clone: 22F6
Regulatory Status: RUO
Other Names: IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Isotype: Armenian Hamster IgG