Biolegend, 137402, Purified anti-mouse Ly49G Antibody, 500μg

Ly49G is a type II transmembrane protein belonging to the family of Ly49 proteins, each having specificity for a small repertoire of MHC I alleles that are generally recognized in a peptide-independent manner.   The Ly49 family of MHC class I receptors shows a high degree of variability between mouse strains.  It is expressed on a subset of NK cells and some NKT cell lines.  The AT8 antibody reacts with the Ly49G receptor from BALB/c, 129/J, 129Sv/J, SJL, FVB/N, A/J, C3H/HeJ, C3H/HeNCr, CBA/J, and CBA/NCr mice.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: IL-2-activated 129/J NK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Makrigiannis AP, et al. 2004. J. Immunol. 172:1414.
RRID: AB_10551620 (BioLegend Cat. No. 137402)
Structure: A type II transmembrane protein belonging to Ly49 family.
Distribution: Subset of NK cells and some NKT cell lines
Function: Allele-specific MHC recognition
Ligand/Receptor: MHC class I molecules
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: Lavender KJ, et al. 2006. J. Immunol. 177:8578. Pascal V, et al. 2007. J. Immunol.. 179:1751. Lavender KJ, et al. 2007. J. Immunol. 179:6856. Rouhi A, et al. 2007. Mol. Immunol. 44(5):821.
Gene ID: 16638
UniProt: View information about Ly49G on UniProt.org
Clone: AT8
Regulatory Status: RUO
Other Names: Ly-49G, Ly49g, Killer cell lectin-like receptor 7, Klra7
Isotype: Mouse IgG2a, κ