Biolegend, 137612, Brilliant Violet 421™ anti-mouse CD335 (NKp46) Antibody, 50μg

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: NKP46-IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.
Application References(PubMed link indicates BioLegend citation): Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ) Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ) Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations: Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed Pierson M, et al. 2021. Curr Protoc. 1:e53. PubMed Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed Serra M, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:609. PubMed Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed Luo B, et al. 2022. Oxid Med Cell Longev. 2022:8965903. PubMed Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed Zindl CL, et al. 2022. Immunity. 55:494. PubMed Ghaffari S, et al. 2022. J Immunol. 208:2246. PubMed Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed LaMarche NM, et al. 2020. Cell Metabolism. 32(2):243-258.e6. PubMed Huggins MA, et al. 2019. Cell Rep. 28:1729. PubMed Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed Shen JZ, et al. 2020. Cell. 184(2):352-369.e23. PubMed Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed Muthalagu N, et al. 2020. Cancer Discov. 1.022222222. PubMed Goh W, et al. 2020. Cell Rep. 33:108285. PubMed Martomo SA, et al. 2021. Mol Cancer Ther. 1.074305556. PubMed Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed Kissiov DU, et al. 2022. Elife. 11:. PubMed Wang X, et al. 2021. Cell. 184:5357. PubMed Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed Wagner AK, et al. 2022. iScience. 25:105137. PubMed Lai NY, et al. 2020. Cell. 180:33:00. PubMed
RRID: AB_10915472 (BioLegend Cat. No. 137611) AB_2563104 (BioLegend Cat. No. 137612)
Structure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
Function: NK cells activation
Ligand/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60. 2. Caligiuri MA. 2008. Blood 112:461. 3. Colonna M. 2009. Immunity 31:15.
Gene ID: 17086
UniProt: View information about CD335 on UniProt.org
Clone: 29A1.4
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.