Biolegend, 137618, PE/Cyanine7 anti-mouse CD335 (NKp46) Antibody, 100μg

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: NKP46-IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.
Application References(PubMed link indicates BioLegend citation): Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ) Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ) Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations: Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed Tian L, et al. 2022. Nat Cancer. 3:1318. PubMed Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed Liang Y, et al. 2022. Theranostics. 12:7729. PubMed Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed Fueyo-González F, et al. 2022. Immunity. 55:459. PubMed Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed Victorino F, et al. 2015. J Immunol. 195: 4973 - 4985. PubMed Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed Xu B, et al. 2021. Nat Commun. 12:5908. PubMed Frasch S, et al. 2016. J Immunol. 197: 1425 - 1434. PubMed Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed Reinfeld BI, et al. 2021. Nature. 593:282. PubMed Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed Wolf KG, et al. 2022. JVS Vasc Sci. 3:336. PubMed Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed Westrich JA, et al. 2019. Oncogene. 38:7166. PubMed Tian L, et al. 2022. Clin Cancer Res. 28:201. PubMed Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
RRID: AB_11218594 (BioLegend Cat. No. 137617) AB_11219186 (BioLegend Cat. No. 137618)
Structure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
Function: NK cells activation
Ligand/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60. 2. Caligiuri MA. 2008. Blood 112:461. 3. Colonna M. 2009. Immunity 31:15.
Gene ID: 17086
UniProt: View information about CD335 on UniProt.org
Clone: 29A1.4
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Rat IgG2a, κ