Biolegend, 137902, Purified anti-mouse Galectin-9 Antibody, 500μg

Galectin-9 is a mammalian lectin with a molecular weight of 40 kD that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers. It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc); Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts and thymus epithelial cells. It may be retained intracellularly or be transported to the cell surface where it is cleaved, thereby generating a soluble form. Galectin-9 is involved in events such as cell aggregation, cell adhesion, chemotaxis and apoptosis. Importantly for the regulation of the immune response, Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full length recombinant mouse Galectin-9 (M-type)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedICFC, IHC-F, WB - VerifiedELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemical staining on frozen tissue sections, the suggested use is 5-10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 108A2 recognizes the linker peptide of mouse Galectin-9; it does not cross-react with stable Galectin-9 (which is biologically active, genetically engineered, linker-less Galectin-9)3. Additional reported applications (for the relevant formats) include: sandwich ELISA as capture antibody1 and immunohistochemical staining of paraffin2 embedded tissue sections.
Application References(PubMed link indicates BioLegend citation): Tsuboi Y, et al. 2007. Clin. Immunol. 124:221. (ELISA) Personal communication. (IHC-Paraffin) Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
Product Citations: Oh J, et al. 2022. Int J Mol Sci. 23:. PubMed Sudhakar JN, et al. 2020. Nat Commun. 3.434722222. PubMed Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed Baloche V, et al. 2021. Scientific Reports. 11(1):5227. PubMed Rebelo C, et al. 2022. Nat Commun. 13:4135. PubMed
RRID: AB_10568691 (BioLegend Cat. No. 137901) AB_10570500 (BioLegend Cat. No. 137902)
Structure: Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
Distribution: Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells
Function: Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
Ligand/Receptor: Tim-3
Cell Type: Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors
Antigen References: 1. Klibi J, et al. 2009. Blood 113:1957. 2. Seki M, et al. 2008. Clin. Immunol. 127:78. 3. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221. 4. Zhu C, et al. 2005. Nat. Immunol. 6:1245. 5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.
Gene ID: 16859
UniProt: View information about Galectin-9 on UniProt.org
Clone: 108A2
Regulatory Status: RUO
Other Names: Lgals9, Gal-9, Ecalectin
Isotype: Rat IgG2a, κ