Biolegend, 138006, Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin) Antibody, 100μg

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo .
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: VE-cadherin-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.
Application References(PubMed link indicates BioLegend citation): Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block) Liao F, et al. 2000. Cancer Res. 60:6805. (FC) Crosby CV, et al. 2005. Blood 105:2771. (FC) Liao F, et al. 2002. Cancer Res. 62:2567. (IF) May C, et al. 2005. Blood 105:4337. (epitope)
Product Citations: Himburg HA et al. 2018. Cell stem cell. 23(3):370-381 . PubMed Grünewald M, et al. 2021. Science. 373: . PubMed Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed Himburg HA, et al. 2019. J Clin Invest. 130:315. PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Rybtsov S, et al. 2011. J Exp Med. 208:1305. PubMed Kedem A, et al. 2017. J Ovarian Res. 10.1186/s13048-017-0354-z. PubMed Himburg H, et al. 2016. Nat Microbiol. 2:16232. PubMed Poulos M, et al. 2016. Nat Commun. 7:13829. PubMed Lehmann GL, et al. 2020. J Exp Med. 217:00:00. PubMed Dravis C et al. 2018. Cancer cell. 34(3):466-482 . PubMed Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed Zhang J, et al. 2021. Nature. 590:457. PubMed Wei Q, et al. 2020. Dev Cell. 53:503. PubMed Gordon-Keylock S, et al. 2013. Blood. 122:2338. PubMed Renner B, et al. 2013. J Am Soc Nephrol. 24:1849. PubMed Xu C, et al. 2018. Nat Commun. 9:2449. PubMed Gao X, et al. 2021. Nature. 589:591. PubMed Mizoguchi T, et al. 2014. Dev Cell. 29:340. PubMed Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
RRID: AB_10568319 (BioLegend Cat. No. 138005) AB_10569114 (BioLegend Cat. No. 138006)
Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand/Receptor: CD144
Cell Type: Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821. 2. Hirashima M, et al. 2009. Blood 93:1253. 3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. 4. Matsumura K, et al. 2003. Blood 101:1367. 5. Hirashima M, et al. 2009. Blood 101:2261. 6. Gotsch U, et al. 1997. J. Cell Sci. 110:583. 7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.
Gene ID: 12562
UniProt: View information about CD144 on UniProt.org
Clone: BV13
Regulatory Status: RUO
Other Names: Vascular endothelial-cadherin
Isotype: Rat IgG1, κ