Biolegend, 138019, Ultra-LEAF™ Purified anti-mouse CD144 (VE-cadherin) Antibody, 100μg

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo .
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: VE-cadherin-Ig fusion protein
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested WB, ICC, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.
Application Notes: Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.
Application References(PubMed link indicates BioLegend citation): Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block) Liao F, et al. 2000. Cancer Res. 60:6805. (FC) Crosby CV, et al. 2005. Blood 105:2771. (FC) Liao F, et al. 2002. Cancer Res. 62:2567. (IF) May C, et al. 2005. Blood 105:4337. (epitope)
RRID: AB_2888759 (BioLegend Cat. No. 138019) AB_2888759 (BioLegend Cat. No. 138020)
Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand/Receptor: CD144
Cell Type: Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821. 2. Hirashima M, et al. 2009. Blood 93:1253. 3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. 4. Matsumura K, et al. 2003. Blood 101:1367. 5. Hirashima M, et al. 2009. Blood 101:2261. 6. Gotsch U, et al. 1997. J. Cell Sci. 110:583. 7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.
Gene ID: 12562
UniProt: View information about CD144 on UniProt.org
Clone: BV13
Regulatory Status: RUO
Other Names: Vascular endothelial-cadherin
Isotype: Rat IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.