Biolegend, 138505, APC anti-mouse CD24 Antibody, 25μg

CD24 is a 35-45 kD protein, also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells, hematopoietic stem cells, and neurons. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus/spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (FC)
Product Citations: Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed Xia KS, et al. 2023. Bioact Mater. 21:69. PubMed Feng H, et al. 2020. J Clin Invest. 130:6354. PubMed Zhou W, et al. 2019. Cell Syst. 0.597916667. PubMed Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed Jiang G, et al. 2021. Cell Death Dis. 12:979. PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Lu X, et al. 2019. Cell Rep. 28:472. PubMed Shen Y, et al. 2022. Cell Death Dis. 13:749. PubMed Mead BE et al. 2018. BMC biology. 16(1):62 . PubMed Ying Z, et al. 2020. Cell Stem Cell. 26(3):403-419. PubMed Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
RRID: AB_2565650 (BioLegend Cat. No. 138505) AB_2565651 (BioLegend Cat. No. 138506)
Structure: GPI-linked sialoglycoprotein, 35-45 kD.
Distribution: Lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells, and neurons
Function: Regulate B and T cell proliferation and differentiation.
Ligand/Receptor: P-selectin, CD24
Cell Type: B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Lymphocytes, Monocytes, Neurons, Thymocytes
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Li YS, et al. 1993. J. Exp. Med. 178:951. 2. Aigner S, et al. 1995. Int. Immunol. 7:1557. 3. Hunte BE, et al. 1998. Eur. J. Immunol.. 28:3850. 4. Auerbach R, et al. 1996. Stem Cells 14:269. 5. Allman DM, et al. 1993. J. Immunol.. 151:4431.
Gene ID: 12484
UniProt: View information about CD24 on UniProt.org
Clone: 30-F1
Regulatory Status: RUO
Other Names: Heat Stable Antigen (HSA), HsAg, Ly-52, Nectadrin
Isotype: Rat IgG2c, κ