Biolegend, 138507, PE/Cyanine7 anti-mouse CD24 Antibody, 25μg

CD24 is a 35-45 kD protein, also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells, hematopoietic stem cells, and neurons. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus/spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (FC)
Product Citations: Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
RRID: AB_2819878 (BioLegend Cat. No. 138507) AB_2832499 (BioLegend Cat. No. 138508)
Structure: GPI-linked sialoglycoprotein, 35-45 kD.
Distribution: Lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells, and neurons
Function: Regulate B and T cell proliferation and differentiation.
Ligand/Receptor: P-selectin, CD24
Cell Type: B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Lymphocytes, Monocytes, Neurons, Thymocytes
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Li YS, et al. 1993. J. Exp. Med. 178:951. 2. Aigner S, et al. 1995. Int. Immunol. 7:1557. 3. Hunte BE, et al. 1998. Eur. J. Immunol.. 28:3850. 4. Auerbach R, et al. 1996. Stem Cells 14:269. 5. Allman DM, et al. 1993. J. Immunol.. 151:4431.
Gene ID: 12484
UniProt: View information about CD24 on UniProt.org
Clone: 30-F1
Regulatory Status: RUO
Other Names: Heat Stable Antigen (HSA), HsAg, Ly-52, Nectadrin
Isotype: Rat IgG2c, κ