Biolegend, 139204, PE anti-mouse PDC-TREM Antibody, 100μg

PDC-TREM, also known as plasmacytoid dendritic cell (PDC) -specific receptor, is a member of the TREM receptor family. It is a transmembrane protein composed of an extracellular region with one V-type Ig domain, a transmembrane region with a lysine residue, and a cytoplasmic domain with no recognized signaling motifs. The lysine present in the transmembrane region allows PDC-TREM to form a complex with DAP12 and Plexin-A1. PI3K and Erk 1/2 are involved in the signal transduction of this complex. PDC-TREM is expressed in TLR-activated PDCs and is involved in the production of IFN-α.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant soluble PDC-TREM
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blot1.
Application References(PubMed link indicates BioLegend citation): Watarai H, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2993. (WB)Rahim MM, et al. 2013. J. Immunol. 190:3994. PubMed.
Product Citations: Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed Rahim M, et al. 2013. J Immunol. 190:3994. PubMed
RRID: AB_10613646 (BioLegend Cat. No. 139203) AB_10613471 (BioLegend Cat. No. 139204)
Structure: Member of the TREM receptor family. Type I protein composed of an extracellular region with a single V-type Ig domain, a transmembrane domain with a lysine residue and a cytoplasmic domain with no recognized signaling motifs.
Distribution: Expressed on TLR activated plasmacytoid dendritic cells (PDC)
Function: Signal transduction, induces the production of IFN-α
Interaction: DAP12, Plexin-A1, PI3K, Erk 1/2
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Jill W, et al. 2009. Curr. Opin. Immunol. 21:38. 2. Lanier LL. 2009. Immunol. Rev. 227:150.
Gene ID: 328830
UniProt: View information about PDC-TREM on UniProt.org
Clone: 4A6
Regulatory Status: RUO
Other Names: Plasmacytoid dendritic cell-specific receptor
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.