Biolegend, 139308, PerCP/Cyanine5.5 anti-mouse CD64 (FcγRI) Antibody, 100μg

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
Application References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed van Wijck Y, et al. 2018. J Immunol. 200:1570. PubMed Vaillancourt M, et al. 2023. PLoS Pathog. 19:e1010942. PubMed Maccio-Maretto L, et al. 2022. Immunology. 167:64. PubMed Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed Caslin HL, et al. 2023. Front Immunol. 13:984859. PubMed Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed Pisu D, et al. 2020. STAR Protoc. 1:100123. PubMed De Schepper S et al. 2018. Cell. 175(2):400-415 . PubMed Dodagatta-Marri E, et al. 2021. Cell Rep. 36:109309. PubMed Marinaik CB, et al. 2020. Cell Rep Med. 1:100095. PubMed Baasch S, et al. 2021. Cell. . PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed Pisu D, et al. 2020. Cell Rep. 30:335. PubMed Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed Lee W, et al. 2021. J Virol. 95:e0053021. PubMed Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed Shibata T, et al. 2020. J Clin Invest. 130:3021. PubMed Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed Sung PS, et al. 2022. JCI Insight. :. PubMed Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed Hakim R, et al. 2021. J Neurosci. 41:8441. PubMed Feuerstein R, et al. 2015. J Immunol. 194:2735. PubMed Kolter J, et al. 2016. J Immunol. 196: 2733 - 2741. PubMed Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed Subramanian S, et al. 2022. Nat Immunol. 23:458. PubMed Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed Ge Y, et al. 2020. Mucosal Immunol. 13:34. PubMed Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed
RRID: AB_2561962 (BioLegend Cat. No. 139307) AB_2561963 (BioLegend Cat. No. 139308)
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, mast cells, dendritic cells
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG
Cell Type: Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Gene ID: 14129
UniProt: View information about CD64 on UniProt.org
Clone: X54-5/7.1
Regulatory Status: RUO
Other Names: FcRI
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.