Biolegend, 139309, Brilliant Violet 421™ anti-mouse CD64 (FcγRI) Antibody, 50μg

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.75 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
Application References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Liu J, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.139. PubMed Komuczki J, et al. 2019. Immunity. 50:1289. PubMed Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed Gravina A, et al. 2023. Nat Biotechnol. 41:717. PubMed Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed De C, et al. 2023. JCI Insight. 8:. PubMed Cansever D, et al. 2023. Nat Immunol. . PubMed Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed Fontana MF, et al. 2023. Elife. 12: . PubMed Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Chikina AS, et al. 2020. Cell. 183:411. PubMed Zhang H, et al. 2017. Leukemia. 10.1128/mBio.00226-17. PubMed Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed Liu J, et al. 2020. Am J Pathol. 190:1298. PubMed Zhou Y, et al. 2020. Immunity. 52(2):357-373. PubMed Kovács B, et al. 2020. Front Immunol. 2.508333333. PubMed Kedage V, et al. 2022. MAbs. 14:2040083. PubMed Ku MW, et al. 2020. Cell Host Microbe. 29(2):236-249.e6. PubMed Zhang S, et al. 2019. Cell Metab. 29:443. PubMed Schippers A, et al. 2015. Mucosal Immunol. 101038/mi201582. PubMed Matheis F, et al. 2020. Cell. 180(1):64-78.e16.. PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed Branchett WJ, et al. 2020. Wellcome Open Research. 0.278472222. PubMed Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed Draheim M, et al. 2017. EMBO Mol Med. 1.489583333. PubMed Riding AM, et al. 2022. iScience. 25:104660. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Martinez-Turtos A, et al. 2022. Oncoimmunology. 11:2116844. PubMed Wang Y, et al. 2020. eLife. 9:e57438.. PubMed Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Liu J, et al. 2018. Am J Pathol. 188:2786. PubMed Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed Silva HM, et al. 2019. J Exp Med. 216:786. PubMed Sharma M, et al. 2019. Immunometabolism. 1. PubMed Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed Barnette DN, et al. 2018. JCI Insight. 3. PubMed Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed Lai NY, et al. 2020. Cell. 180:33:00. PubMed Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed Jones GS, et al. 2020. mSphere. 5:. PubMed Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed Rivera CA, et al. 2022. Immunity. 55:129. PubMed Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed Fearon AE, et al. 2021. iScience. 24:103143. PubMed
RRID: AB_2562694 (BioLegend Cat. No. 139309)
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, mast cells, dendritic cells
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG
Cell Type: Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Gene ID: 14129
UniProt: View information about CD64 on UniProt.org
Clone: X54-5/7.1
Regulatory Status: RUO
Other Names: FcRI
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.