Biolegend, 139515, Pacific Blue™ anti-mouse TCR Vβ5.1, 5.2 Antibody, 25μg

Vβ5.1 and 5.2 T cell receptor (TCR Vβ5.1, 5.2) are variants of TCR β chain that, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of T cell-mediated immune responses.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Murine T cell hybridoma 2HB51.8
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Induction of proliferation of Vß5.1+ and Vß5.2+ T cells2, 3 and in vivo depletion of Vß5+ T cells4.
Application References(PubMed link indicates BioLegend citation): Kanagawa O, et al. 1991. J. Immunol. 147:1307. (FC) Kanagawa O, et al. 1992. J. Immunol. 149:9. (Activ) Woodland DL, et al. 1993. J. Exp. Med. 177:433. (Activ) Gelber C, et al. 1992. Cancer Res. 52:6507. (Deplete)
Product Citations: Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed Guo R, et al. 2020. Cell Res. 30:21. PubMed Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed Meyers JL, et al. 2018. PLoS One. 13:e0207007. PubMed Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
RRID: AB_2750134 (BioLegend Cat. No. 139515) AB_2750135 (BioLegend Cat. No. 139516)
Structure: Member of the immunoglobulin superfamily, Vβ5.1and 5.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution: Expressed on a subset of TCR αβ+ T cells.
Function: Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
Ligand/Receptor: MHC-Peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171. 2. Sim GK and Augustin AA. 1985. Cell 42:89. 3. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114.
Gene ID: 21577
UniProt: View information about TCR Vbeta5.1 5.2 on UniProt.org
Clone: MR9-4
Regulatory Status: RUO
Other Names: Vβ5 T-cell receptor, T cell receptor beta 5
Isotype: Mouse IgG1, κ