Biolegend, 140207, APC anti-mouse CD157 (BST-1) Antibody, 25μg

CD157 is an ectoenzyme member of the ADP-ribosyl cyclase family, also known as ADP-ribosyl cyclase 2, BST-1 (bone marrow stromal cell antigen 1), BP-3 antigen. It has a molecular weight between 40 - 46 kD and is a GPI-linked cell membrane glycoprotein which is expressed as a homodimer. CD157 is expressed by macrophages, neutrophils, bone marrow stromal cells, endothelial cells, follicular dendritic cells, and T and B cell progenitors prior to the rearrangement of the antigen receptors. Besides the ectoenzyme activity, it also plays a role in neutrophil migration and adhesion.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Murine pre-B cell line 18.81
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): McNagny KM, et al. 1988. J. Immunol. 141:2551. (FC)
Product Citations: Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed Lütge M, et al. 2023. Nat Immunol. . PubMed Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed Wakabayashi T, et al. 2022. Invest Ophthalmol Vis Sci. 63:5. PubMed Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
RRID: AB_10900819 (BioLegend Cat. No. 140207) AB_10901172 (BioLegend Cat. No. 140208)
Structure: Member of the ADP-ribosyl cyclase family, ectoenzyme, 40-46 kD, glycosyl-phosphatidylinositol (GPI)- anchored membrane protein, present on the cell surface as a homodimer
Distribution: Expressed by endothelial cells, follicular dendritic cells, cells from the myeloid lineage, and T and B cell precursors prior to the rearrangement of the antigen receptor. Expressed in many cell types from different tissues like the bone marrow, spleen, thymus, lung, kidney and heart.
Function: Synthesizes cyclic ADP-ribose (cADPR), a second messenger that elicits calcium release from intracellular stores.
Cell Type: B cells, Dendritic cells, Endothelial cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Yamamoto-Katayama S, et al. 2002. J. Mol. Biol. 310:711. 2. Funaro A, et al. 2009. Front. Biosci. 14:929. 3. Malavasi F, et al. 2006. Mol. Med. 12:334. 4. Vicari AP, et al. 1996. Int. Immunol. 8:183.
Gene ID: 12182
UniProt: View information about CD157 on UniProt.org
Clone: BP-3
Regulatory Status: RUO
Other Names: cADPr hydrolase 2, Ly-65, BP-3, ADP-ribosyl cyclase 2
Isotype: Mouse IgG2b, κ