Biolegend, 140306, Pacific Blue™ anti-mouse CD90.2 (Thy1.2) Antibody, 100μg

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).
Application References(PubMed link indicates BioLegend citation): Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC) Jameson J, et al. 2004. J. Immunol. 172:3573. (IF) Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
Product Citations: Dalmas E et al. 2017. Immunity. 47(5):928-942 . PubMed Pourebrahim R, et al. 2023. Cell Death Dis. 14:371. PubMed Hu X, et al. 2016. Nat Commun. 7:13095. PubMed Khodeer S, et al. 2022. J Cell Sci. 135: . PubMed Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed Mathur S, et al. 2021. JCI Insight. 6:. PubMed Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed Mohammed RN, et al. 2019. Sci Rep. 4.185416667. PubMed Deets KA, et al. 2021. Elife. 10:. PubMed
RRID: AB_10645335 (BioLegend Cat. No. 140305) AB_10641693 (BioLegend Cat. No. 140306)
Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand/Receptor: CD45
Cell Type: Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules
Antigen References: 1. Borrello M, et al. 1996. Cell. Immunol.173:198. 2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220. 3. Williams A, et al. 1982. Science 216:696.
Gene ID: 21838
UniProt: View information about CD90.2 on UniProt.org
Clone: 53-2.1
Regulatory Status: RUO
Other Names: Thy-1.2
Isotype: Rat IgG2a, κ