Biolegend, 141116, Brilliant Violet 421™ anti-mouse TCR Vγ1.1/Cr4 Antibody, 50μg

T cell receptor (TCR) is a heterodimer consisting of an α and β chain (TCR α/β) or a γ and δ chain (TCR γ/δ). TCR associates with CD3 to form a CD3/TCR complex. The CD3/TCR plays a key role in antigen recognition, signal transduction, and T cell activation. TCR Vγ1.1 (Garman nomenclature) is also called TCR Vγ1 (Tonegawa nomenclature). The Vγ1 gene almost exclusively rearranges to the Jγ4-Cγ4 gene. Vγ1- Jγ4-Cγ4 expressing cells constitute a major population of γ/δ T cells in the thymus and peripheral lymphoid organs in adult mice, but they are only composed of a minor population of γ/δ T cells during fetal and early postnatal life. Vγ1 T cell development can happen in thymus-dependent and thymus-independent manners. Further studies have shown that the antibody 2.11 recognizes an epitope located in the Cr4 domain. Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts .
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: T3.13.1 T-cell hybridoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Pereira P, et al. 1995. J. Exp. Med. 182:1921. (IP) Pereira P and Boucontet L. 2004. J. Immunol. 173:3261. (FC) Pereira P, et al. 1996. Intl. Immunol. 8:83. (FC)
RRID: AB_3106198 (BioLegend Cat. No. 141116)
Structure: Ig superfamily
Distribution: Subset of γ/δ T-cells
Function: Antigen recognition
Ligand/Receptor: Antigen
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Pereira P, et al. 1995. J. Exp. Med. 182:1921. 2. Grigoriadou K, et al. 2002. J. Immunol. 169:3736.
Gene ID: 110067
UniProt: View information about TCR Vgamma1.1/Cr4 on UniProt.org
Clone: 2.11
Regulatory Status: RUO
Other Names: T cell receptor Vgamma 1.1, TCR Vγ1 (Tonegawa nomenclature), TCR Cr4, TRGV1, GV5S1
Isotype: Armenian Hamster IgG
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.