Biolegend, 141402, Purified anti-mouse LAP (TGF-β1) Antibody, 100μg

Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associate peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse Tgfb1-transduced P3U1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-ß, and pro-TGF-ß. Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB) Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
Product Citations: Widjaja AA, et al. 2019. Gastroenterology. 157:777. PubMed Wang W, et al. 2018. Cancer Cell. 34:757. PubMed Steenbrugge J, et al. 2018. J Exp Clin Cancer Res. 37:191. PubMed Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed Mencarelli A, et al. 2018. Front Immunol. 9:261. PubMed Nawaz A, et al. 2018. Sci Rep. 6:37. PubMed Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed Škuljec J, et al. 2017. Front Immunol. 1.114583333. PubMed Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
RRID: AB_10719836 (BioLegend Cat. No. 141402)
Structure: Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Distribution: Many cell types, highly expressed on activated Tregs and platelets
Function: TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
Ligand/Receptor: TGF-β receptors
Cell Type: Dendritic cells, Platelets, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Oida T, et al. 2010. PLoS One 5:e15523. 2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445. 3. Ochi H, et al. 2006. Nat. Med. 12:627. 4. Oida T, et al. 2003. J. Immunol. 170:2516. 5. Nakamura K. 2001. J. Exp. Med. 194:629. 6. Miyazono K, et al. 1993. Growth Factors 8:11.
Gene ID: 21803
UniProt: View information about LAP on UniProt.org
Clone: TW7-16B4
Regulatory Status: RUO
Other Names: Latency Associated Peptide (LAP), Transforming growth factor-beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
Isotype: Mouse IgG1, κ