Biolegend, 142804, PE anti-mouse CD159a (NKG2A, 200μg

CD159a, also known as NKG2A or KLRC1 (killer cell lectin-like receptor subfamily C, member 1), is a 43 kD type II transmembrane protein with extracellular C-type lectin domains.  It belongs to the killer cell lectin-like receptor family also known as the NKG2 family.  It is expressed on NK and NKT cells and activated CD8+ T cells.  NKG2A binds to non-classical MHC class I molecule Qa-1 and causes inhibition of NK cell-mediated target-cell lysis.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C57BL/6 mouse CD94/NKG2A transfected CHO cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use licenses cannot be guaranteed from the University of California.
Application References(PubMed link indicates BioLegend citation): McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC) Vance RE, et al. 2002. P. Natl. Acad. Sci. USA 99:868. (FC)
Product Citations: Firouzi J, et al. 2023. Cell J. 25:92. PubMed Li Y, et al. 2014. PLoS One. 9:86927. PubMed Alvarez M, et al. 2020. Cancers (Basel). 12:. PubMed Kissiov DU, et al. 2022. Elife. 11:. PubMed Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
RRID: AB_10959654 (BioLegend Cat. No. 142803) AB_10965542 (BioLegend Cat. No. 142804)
Structure: Type II transmembrane protein with extracelluar C-type lectin domains, complexed with CD94; 43 kD
Distribution: NK cells and NK T cells from C57BL/6 mouse
Function: Inhibitory activity via ITIMs
Ligand/Receptor: Non-classical MHC-I molecule Qa-1
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells, NK cells, NKT cells
Biology Area: Cell Biology, Immunology, Signal Transduction, Stem Cells
Molecular Family: CD Molecules, MHC Antigens
Antigen References: 1. Vance RE, et al. 1998. J Exp Med. 188:1841-8. 2. Lohwasser S, et al. 1999. Eur J Immunol. 29:755-61. 3. Sivakumar PV, et al. 1999. J Immunol. 162:6976-80. 4. Silver ET, et al. 1999. Immunogenetics 49:727-30. 5. Vance RE, et al. 1999. J Exp Med. 190:1801-12.
Gene ID: 16641
UniProt: View information about CD159a on UniProt.org
Clone: 16A11
Regulatory Status: RUO
Other Names: KLRC1, NKG2B, NKG2AB6
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.