Biolegend, 144405, PE anti-mouse DR3 (TNFRSF25) Antibody, 25μg

DR3, also known as TNFRSF25, lymphocyte-associated receptor of death (LARD), WSL-1, TRAMP, TR3 and APO3, is a 55 kD glycoprotein belonging to the TNF receptor superfamily (TNFRSF). TNFSF25 is expressed at low level on CD4-positive, CD8-positive T cells and NKT cells. It has been shown to stimulate NF-κB activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domains containing adaptor proteins. Recently, it has been shown that TNFRSF25 is also expressed on Treg cells and regulates Treg proliferation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mtnfr25-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: 4C12 is an agonistic antibody. It leads to rapid and selective proliferation of pre-existing Tregs in vivo through TCR engagement with MHC class II (MHC-II) and IL-2 signaling. We recommend our Ultra-LEAF™ purified 4C12 antibody (Cat. No. 144411 and 144412) for agonist or in vivo studies.
Application References(PubMed link indicates BioLegend citation): Fang L, et al. 2008. J. Exp. Med. 205:1037. Wolf D, et al. 2012. Transplantation. 94:569. PubMed Schreiber TH, et al. 2012. J. Immunol. 189:3311. Longman RS, et al. 2014. J Exp Med. 211:1571. PubMed
Product Citations: Castellanos JG et al. 2018. Immunity. 49(6):1077-1089 . PubMed Longman R, et al. 2014. J Exp Med. 211:1571. PubMed
RRID: AB_2561688 (BioLegend Cat. No. 144405) AB_2561689 (BioLegend Cat. No. 144406)
Structure: 55 kD glycoprotein, contains one death domain and four TNFR-Cys repeats
Distribution: CD4, CD8, NKT and Tregs at low level
Function: Plays a role in lymphocyte homeostasis, induces NFKB activation and apoptosis
Ligand/Receptor: TL1-A
Cell Type: NKT cells, T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Taraban VY, et al. 2011. J. Clin. Invest. 121:463. Slebioda TJ, et al. 2011. Eur. J. Immunol. 41:2606. Schreiber TH, et al. 2010. J. Clin. Invest. 120:3629. Reddy PB, et al. 2012. J. Virol. 2012. [Epub].
Gene ID: 85030
UniProt: View information about DR3 on UniProt.org
Clone: 4C12
Regulatory Status: RUO
Other Names: Apo-3, LARD, TNFR25, TRAMP, WSL-1, DR3
Isotype: Armenian Hamster IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.