Biolegend, 144702, Purified anti-mouse Ly49H Antibody, 200μg

Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein.  It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not Balb/c or DBA/2 mice).  Expression of Ly49H has been associated with resistance to MCMV infection in C57BL/6 mice.  Modulation of Ly49H expression by its antibody made MCMV-resistant C57BL/6 mice susceptible to infection.  Unlike Ly49A, Ly49H lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) but has ITAM (immunoreceptor tyrosine-based activation motif), which is associated with DAP-12 (KARAP, killer cell activating motif).
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C1498-Ly49A/H transfectant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats of this clone) include: immunoprecipation1, and in vitro2 and in vivo3 blocking activity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 144717 & 144718).
Application References(PubMed link indicates BioLegend citation): Guseva NV, et al. 2010. Eur. J. Immunol. 40:2618. (IP) Orr MT, et al. 2009. J. Exp. Med. 206:807. (Block) Rodriguez M, et al. 2004. J. Immunol. 173:6312. (Block)
Product Citations: Potempa M, et al. 2022. J Immunol. 208:1742. PubMed Niogret C, et al. 2019. Nat Commun. 10:1444. PubMed Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed Goh W, et al. 2020. Cell Rep. 33:108285. PubMed Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
RRID: AB_2561549 (BioLegend Cat. No. 144702)
Structure: 28.3 kD
Distribution: NK cells but not NKT cells
Function: Interacts with m157, a cytomegalovirus-encoded protein
Ligand/Receptor: m157
Cell Type: NK cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165. 2. Lee SH, et al. 2001. Nat. Genet. 28:42. 3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.
Gene ID: 16639
UniProt: View information about Ly49H on UniProt.org
Clone: 3D10
Regulatory Status: RUO
Other Names: Klra8, Cmv1
Isotype: Mouse IgG1, κ