Biolegend, 145209, Brilliant Violet 421™ anti-mouse CD304 (Neuropilin-1) Antibody, 125microl

CD304, also known as neuropilin-1, is a 140 kD type I transmembrane protein. Its extracellular region contains two CUB, two FV/FVIII, and one MAM domain. It is expressed by natural regulatory T cells (nTreg), a subset of invariant natural killer T cells (iNKT), endothelial cells, and neurons. Neuropilin-1 stabilizes the interaction between Tregs and dendritic cells, contributes to the recruitment of tumor-infiltrating Tregs in response to tumor-derived VEGF, and influences the process of angiogenesis and axon guidance. The ligands of CD304 are VEGF165 and semaphorin-3A.
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Extracellular region of mouse CD304
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed Verhagen J, et al. 2014. PLoS One. 9e:108023. (FC) PubMed
Product Citations: Brown HM, et al. 2018. Sci Rep. 1.801388889. PubMed Martens PJ, et al. 2022. Front Endocrinol (Lausanne). 13:1023264. PubMed Huang W, et al. 2014. J Immunol. 193:2267. PubMed Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed Yue X, et al. 2019. Nat Commun. 10:2011. PubMed Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed Sharma M, et al. 2020. Circ Res. 127:335. PubMed Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed Matsuda T, et al. 2022. iScience. 25:105324. PubMed Sun F, et al. 2022. Cell Death Dis. 13:181. PubMed Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed Bastow CR, et al. 2022. Front Immunol. 13:873586. PubMed
RRID: AB_2562358 (BioLegend Cat. No. 145209)
Structure: Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
Distribution: Natural regulatory T cells (nTreg), subset of invariant natural killer T cells (iNKT), endothelial cells, neurons
Function: Angiogenesis, axon guidance, modulation of the immune response
Ligand/Receptor: VEGF165, semaphorin-3A
Cell Type: Dendritic cells, Endothelial cells, Neurons, NKT cells, Tregs
Biology Area: Angiogenesis, Cancer Biomarkers, Cardiovascular Biology, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family: Adaptor Proteins, Adhesion Molecules, CD Molecules
Antigen References: 1. Yadav M, et al. 2012. J. Exp. Med. 209:1713. 2. Weiss JM, et al. 2012. J. Exp. Med. 209:1723. 3. Hansen W, et al. 2012. J. Exp. Med. 209:2001. 4. Milpied P, et al. 2011. Blood 118:2993.
Gene ID: 18186
UniProt: View information about CD304 on UniProt.org
Clone: 3E12
Regulatory Status: RUO
Other Names: NRP-1, VEGFR165 receptor
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.