Biolegend, 145806, PE/Cyanine7 anti-mouse PLZF Antibody, 100μg

PLZF (promyelocytic leukemia zinc finger), also known as Zbtb16, Zinc finger and BTB domain-containing protein 16, Zfp145, is a member of the BTB-POZ family of transcription factors. It was first identified in a patient with acute promyelocytic leukemia, where a reciprocal chromosomal translocation t (11;17)(q23;q21) resulted in a fusion with RARA gene encoding retinoic acid receptor alpha. Expression of this transcriptional repressor in immune cells differs between human and mouse. In humans, PLZF is expressed in CD34 + progenitor cells and in primitive multipotent hematopoietic cell lines, NK cells, γ/δ T cells, CD4 + and CD8 + T cells. It is also expressed in MR1-specific mucosal-associated invariant T cells as well as in MHC class II-restricted T cells that develop via a thymocyte-thymocyte interaction. PLZF is involved in NK cell function, cellular quiescence, and growth suppression. It also inhibits gene expression induced by retinoic acid receptor. In mice, PLZF is highly expressed in immature CD1d-resricted invariant NKT (iNKT) cells, a subset of γ/δ (Vg1.1 + Vd6.3 + ) T cells, and non-invariant CD1d-restricted T cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells. It is also reported to be expressed in embryonic tissues, giving rise to hematopoietic progenitors.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Combination of peptides covering the amino, carboxyl and hinge regions of the PLZF.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2566164 (BioLegend Cat. No. 145805) AB_2566165 (BioLegend Cat. No. 145806)
Structure: BTB domain-containing
Distribution: iNKT cells, γ/δ (Vg1.1+Vd6.3+) T cells, non-invariant CD1d-restricted T cells
Function: Involved in NKT cell development
Cell Type: Mesenchymal Stem Cells, NKT cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction, Stem Cells, Transcription Factors
Antigen References: 1. Constantinides MG, et al. 2011. J. Immunol. 187:309. 2. Kovalovsky D, et al. 2008. Nat. Immunol. 9:1055. 3. Chen Z, et al. 1993. EMBO J. 12:1161. 4. Chen Z, et al. 1994. Proc. Natl. Acad. Sci. USA 91:1178.
Gene ID: 235320
UniProt: View information about PLZF on UniProt.org
Clone: 9E12
Regulatory Status: RUO
Other Names: Promyelocytic leukemia zinc finger, Zbtb16, Zinc finger and BTB domain-containing protein 16, Zfp145
Isotype: Armenian Hamster IgG