Biolegend, 146002, Ultra-LEAF™ Purified anti-Asialo-GM1 Antibody, 1ml

GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1 + . The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.
1ml
Verified Reactivity: Mouse, Rat
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Asialo-GM1
Formulation: Lyophilized powder.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: Reconstitute with 1 ml sterile deionized, distilled, endotoxin-free water. Gamma globulin fraction of serum was precipitated using 50% ammonium sulfate followed by dialysis with phosphate buffered saline.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested Depletion - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Optimal staining for flow cytometry may vary from lot-to-lot. This antibody has been reported to deplete NK cells in vivo. A usage of 50 µl per mouse is recommended, but optimal usage will vary depending on the strain of mouse and experimental conditions2-4. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2. This antibody can partially block IL-12 induced IFN-γ production but does not affect other systemic action of IL-12. This product may contain other non-IgG subtypes.
Application References(PubMed link indicates BioLegend citation): Naiki M, et al. 1974. J. Immunol. 113:84. Kasai M, et al. 1981. Nature. 291:334. (Depletion) Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion) Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
Product Citations: Wang X, et al. 2022. Viruses. 14:. PubMed Hu J, et al. 2015. J Virol. 89: 10383 - 10398. PubMed Böttcher JP, et al. 2018. Cell. 172:1022. PubMed Li P, et al. 2020. Nat Commun. 3.504861111. PubMed André P et al. 2018. Cell. 175(7):1731-1743 . PubMed Lin Q, et al. 2021. Nat Commun. 12:2537. PubMed Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed Dai HS et al. 2017. Immunity. 47(1):159-170 . PubMed Gauthier L, et al. 2019. Cell. 177:1701. PubMed Pelly VS, et al. 2021. Cancer Discov. 11:2602. PubMed Bonavita E, et al. 2020. Immunity. 1215:53. PubMed Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed Manangeeswaran M, et al. 2020. Front Immunol. 11:237. PubMed Ma Q, et al. 2021. Precis Clin Med. 4:155. PubMed
RRID: AB_2562206 (BioLegend Cat. No. 146002)
Structure: Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer
Distribution: NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons
Function: Supports cellular identification, neuronal plasticity, and repair mechanisms
Interaction: Facilitates cellular recognition and cell-to-cell communication
Ligand/Receptor: Ligand for cholera toxin
Cell Type: Basophils, Neurons, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References: 1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.
Gene ID: NA
Clone: Poly21460
Regulatory Status: RUO
Other Names: Ganglioside, GM1, ganglio-N-tetraosylceramide
Isotype: Rabbit Polyclonal IgG
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.