Biolegend, 146306, PE anti-mouse IL-17RB Antibody, 100μg

IL-17RB, also known as IL17RH1, belongs to the cytokine receptor family.  IL-17RB possesses a unique intracellular signaling molecule called SEFIR, and is expressed similarily to fibroblast growth factor genes and IL-17R.  It is reported to be expressed on iNKT cells, innate lymphoid cells (ILC), and Th2 cells.  It binds IL-17B and IL-17E (IL-25) but not IL-17A or C.  Its interaction with IL-25 has a higher affinity than that with IL-17B.  Binding of IL-17RB and IL-25/IL-17E induces NF-kB mediated IL-8 production through interaction with TRAF6 and Act1.  IL-17RB has been reported to play a role in autoimmune diseases such as rheumatoid arthritis and asthma.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse IL-17RB - Fc chimera (human IgG1)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations: Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed Matsubara K, et al. 2016. Hypertens Res. 10.1038/hr.2015.160. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
RRID: AB_2564511 (BioLegend Cat. No. 146305) AB_2564512 (BioLegend Cat. No. 146306)
Structure: Single pass type I transmembrane protein, 55.9 kD
Distribution: iNKT cells, innate lymphoid cells, Th2 cells
Function: Binds IL-25 with higher affinity than IL-17B, can block IL-25 mediated pathology
Interaction: TRAF6 and Act1
Ligand/Receptor: IL-25/IL-17E
Cell Type: NKT cells, Th2
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Hwang SY, et al. 2004. Arthritis Res. Ther. 6:R120. 2. Rickel EA, et al. 2008. J. Immunol. 181:4299. 3. Terashima A, et al. 2008. J. Exp. Med. 205:2727.
Gene ID: 50905
UniProt: View information about IL-17RB on UniProt.org
Clone: 9B10
Regulatory Status: RUO
Other Names: Evi27, IL-17ER, IL17RH1, IL17br, IL-17 receptor B
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.