Biolegend, 147306, Alexa Fluor® 594 anti-mouse/human CD324 (E-Cadherin) Antibody, 100μg

CD324, also known as E-cadherin, cadherin-1, CDH1, and UVO is a member of the cadherin superfamily. It is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase-8, β-catenin, presenilin 1, and casein kinase II as well as other extracellular proteins including the EGF receptor.
100μg
Verified Reactivity: Mouse, Human
Reported Reactivity: Cynomolgus, Dog, Pig
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E-Cadherin extracellular domain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested IHC-P, IHC-F, 3D IHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 20 μg/mL is recommended. For immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 μg/mL is suggested. For immunohistochemical staining of frozen tissue sections, a concentration range of 2.0 - 5.0 μg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Application Notes: Additional reported applications (for relevant formats) include: immunoprecipitation1, Western Blotting1, immunomicroscopy3, biological function1,2, and spatial biology (IBEX)4,5.
Application References(PubMed link indicates BioLegend citation): Vestweber D, et al. 1985. EMBO. 4:3393. (IP, WB, FA) Nakagawa M, et al. 2001. J. Cell Sci. 114:1829. (FA in canine cells) Mohamet L, et al. 2010. PLoS ONE. 5:e12921. (IF) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Huber M, et al. 2023. Nat Commun. 14:2143. PubMed Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed Zhang X, et al. 2019. iScience. 19:607. PubMed Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
RRID: AB_2563230 (BioLegend Cat. No. 147306)
Structure: Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Distribution: Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells.
Function: Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization.
Interaction: Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
Ligand/Receptor: αEβ7 integrin.
Cell Type: Embryonic Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Overduin M, et al. 1995. Science 267:386. 2. Boggon TJ, et al. 2002. Science 296:1308. 3. Berx G, et al. 1995. EMBO J. 14:6107. 4. Perl AK, et al. 1998. Nature 392:190.
Gene ID: 99912550
UniProt: View information about CD324 on UniProt.org
Clone: DECMA-1
Regulatory Status: RUO
Other Names: E-Cadherin, Cadherin-1, CDH1, and UVO
Isotype: Rat IgG1, κ