Biolegend, 148202, Purified anti-mouse/rat XCR1 Antibody, 100μg

XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α + conventional dendritic cells capable of antigen cross-presentation.
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.
Application References(PubMed link indicates BioLegend citation): Gurka S, et al. 2015. Front Immunol. 6:35. (FC) Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
Product Citations: Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed Oba T, et al. 2021. J Immunother Cancer. 9:. PubMed Liu H, et al. 2020. J Immunol. 205:1207. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed Maier B, et al. 2020. Nature. 580:257. PubMed
RRID: AB_2563841 (BioLegend Cat. No. 148202)
Structure: Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution: Subset of CD8α+ conventional dendritic cells.
Function: Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
Ligand/Receptor: XCL1 and XCL2.
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yamazaki C, et al. 2013. J. Immunol. 190:6071. 2. Shimizu K, et al. 2013. J. Immunol. 190:5609. 3. Bachem A, et al. 2012. Front. Immunol. 3:214. 4. Crozat K, et al. 2011. J. Immunol. 187:4411.
Gene ID: 23832301086
UniProt: View information about XCR1 on UniProt.org
Clone: ZET
Regulatory Status: RUO
Other Names: GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype: Mouse IgG2b, κ